Fig. 1

Malignant involvement of the serous body cavities is associated with converting M1-like TAMs to M2-like TAMs. (A) Representative composite of the m-IHC panel. Scale bar: 100 μm. (B) Distinct distributions of TAM population densities across regions in early stage (n = 16), late stage (n = 16), and omental metastasis (n = 16). (C) Ratio of M1-like polarized MФs (CD86⁺CD68⁺) to M2-like TAMs (CD206⁺/CD163⁺CD68⁺) (n = 16). (D) Ratio of M2-like TAMs to EOC (PAX8⁺) (n = 16). (E) Flow cytometric staining of CD11b⁺CD14⁺CD68⁺CD86⁺ (M1-like TAMs) or CD11b⁺CD14⁺CD68⁺CD206⁺ (M2-like TAMs) ascites TAMs from patients with EOC (n = 10). The data were presented as the means ± SEM and were analyzed by unpaired Student’s t-tests. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001