Fig. 3

Unsupervised analysis of 16s rRNA data identified 4 distinct enterotypes. (A) Dot plot depicting coordinates of each sample on the principal co-ordinate analysis (PCoA) performed using the Bray-Curtis matrix distance. Samples were clustered using hierarchical k-means, with each cluster representing an enterotype. (B) Comparison of the time elapsed from the initiation of chemotherapy in the AML-cohort between the enterotypes. p-value was computed with bilateral Wilcoxon test. (C) Bar plot showing the distribution of the 4 enterotypes among the different clinical timepoints (All AML baseline, AML-C diarrhea (-) at Day 14, AML-C diarrhea (+), AML-NE, and N-AML-NE at diagnosis). Box plots depicting the comparisons of (D) bacterial load (bacteria / g of feces), and (E) Shannon α -diversity index between the 4 enterotypes [enterotype 1 (n = 19), enterotype 2 (n = 31), enterotype 3 (n = 25) and enterotype 4 (n = 18)]. Two tailed p-values were performed using Kruskal-Wallis test with Dunn’s multiple comparisons tests. p-value < 0.01 was considered statistically significant