Fig. 2

Erythroid progenitor cell exhaustion and impaired terminal erythropoiesis are observed in NHD13 mice. Quantification of hematopoietic progenitor cell populations (A) and representative flow cytometric plots (B) in the BM of NHD13 and WT mice at 12 and 20 weeks (n = 5–6 per group). Absolute cell numbers were normalized to a single femur. C–F Comparison of the proportions and absolute cell counts (per femur) of erythroblasts at different stages of terminal erythroid differentiation in the BM of NHD13 and WT mice at 12 (C, D) and 20 weeks (E, F) of age. CD44 levels and forward scatter (FSC) were analysed to define various developmental stages of erythroblasts (n = 8–9 per group). All the data are shown as mean ± s.e.m. A two-tailed unpaired Student t test was performed between means of two groups. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. LSK: Lin⁻Sca-1⁺c-kit⁺ cells; MP: multipotent progenitors; GMP: granulocyte/macrophage progenitors; CMP: common myeloid progenitors; MEP: megakyocyte-erythroid progenitors; ErP: erythroid-committed progenitors (Lin⁻ckit⁺Sca-1⁻CD34⁻Fcγ⁻CD71⁺); MkP: Megakaryocyte-committed progenitors; Bipotential: bipotential progenitors. I: proerythroblasts; II: basophilic erythroblasts; III: polychromatic erythroblasts; IV: orthochromatic erythroblasts; V:reticulocytes; VI: mature red blood cells